Microbiological Media disposal; Purpose
Microbiological Media disposal; To dispose of the used media properly in order to lessen Microbiology Laboratory Contamination as well as environmental pollution.
Scope
This SOP is applicable for the disposal of used Media in the Microbiology Laboratory at XX Pharmaceuticals Ltd.
Definition
N/A
Responsibilities
The roles and responsibilities are as follows:
Laboratory Attendant
Clean & disinfect used media
Executive/ Sr. Executive, Microbiology
- Monitor disposal activity accordingly.
- Follow the instructions of this procedure appropriately.
- Follow the instructions of this procedure properly.
Asst. Manager, Microbiology
- Confirm proper disposal of used media.
- Confirm that this procedure is kept up to date.
- Confirm suitable personnel from the section are trained in this practice.
- Confirm that SOP is technically sound and reflects the required practices.
Head of Quality Assurance
Approval of this SOP
Procedure
Instructions
- Appropriately wear heat-resistant gloves, eye protection, and laboratory coat during handling autoclaved media.
- Avoid autoclaving the sealed containers or completely filled bottles with narrow necks as they may explode.
- Don’t expose any used media container or plate outside the Laminar Air Flow cabinet.
- Wash and disinfect both hands after handling used media.
Collection of Used Media
- Wear suitable garments, gloves, and mask.
- Wear safety goggles if hazardous media are subject to being discarded.
- Collect all the media to be disposed of in the designated vessel after use.
- Close the mouth of the vessel tightly.
Sterilization
- Attach the Autoclave Tap with the vessel.
- Place the vessel into a sterilizer and sterilize at 1210 C & 15 lbs for 30 minutes.
- Check that the color of the autoclave tap is turned black.
Discard Method
- After autoclaving, collect the media in a specific container when the temperature comes down to 500 to 550 C and overlay the media with 40% formaldehyde solution.
- Keep the media for 30 minutes.
- Dispose of the media into the drain which is linked with ETP.
- Rinse the vessel properly with hot water.
- Wash the vessel with detergent.
- Disinfect the whole vessel with 70% Iso Propyl Alcohol and dry the vessel.
Record Maintain:
Maintain Register of Media Disposal Record in Annexure-I.