Micropipette; Purpose
To initiate that the procedure is suitable for ideal operation, cleaning and calibration of Micropipette for use in microbiology test.
Scope
This SOP applies for Operation, Cleaning & Calibration of Micropipette, Model: 8-105-00-9 & 8-106-00-9 in Microbiology Section at XX Pharmaceuticals Limited.
Definitions/Abbreviations
N/A
Responsibilities
The roles and responsibility is as follows
Executive/ Senior Executive, Microbiology
Operation, Cleaning and Calibration of Micropipette.
Manager, Microbiology
- Ensure Operation, Cleaning, Calibration and application of sound technical information.
- Review that SOP is technically informative.
Head of Quality Assurance
Take initiative to Approval of SOP
Procedure:
Instructions
- Keep Micropipette always at vertical position on the respective stand.
- Never sink Micropipette into water.
- Never reset or adjust the volume.
- Care should be taken during pipetting that solutions do not enter into Micropipette.
- Do not try to volume highly viscous solution by this device.
- Do not use hot solution in this device.
Operation Procedure
- Check is calibration status of Micropipette. If Micropipette is not calibrated, calibrate it before use.
- Set desired volume by turning red colored setting screw button at clock wise for increasing and anticlockwise for decreasing the volume.
- Set micropipette tips on bottom side in tight condition.
- After setting desired volume, observe display digit.
- Draw desired volume of solution by pressing red colored setting screw in single punch.
- Rinse tips least three times with solution at first time.
- Deliver total volume by pressing same button at double punch.
- Draw total volume of the solution.
- Press setting screw for delivery the solution.
- After use, always keep it on the stand at vertical position.
Cleaning Procedure
- Clean outer surface with dry cloth.
- Use 70% IPA solution to disinfect outer surfaces except display of Micropipette.
- Clean Micropipette before & after use.
Calibration Procedure
- Set Micropipette reading as 100 µL for Model: 8-105-00-9 & 500 µL for Model: 8-106-00-9.
- Set tips with Micropipette properly.
- Take distilled water of temperature at 250C±10C into a beaker.
- Weigh & tare another 100 ml beaker.
- Draw 100 µL or 500 µL of distilled water by Micropipette & deliver it into tarred beaker.
- Record weight of distilled water.
- Repeat same procedure up to nine times.
- Calculate the accuracy(% of error) & Precision(% CV) as below :
- Accuracy (% error) = Mean Value – Reference value/Reference value x 100
- Precision (% CV) = Standard deviation/mean x 100.
- Record the result in Micropipette Calibration Record, Annexure-I.
- Calibration Frequency: Perform the calibration once in a year.
Maintenance
If Micropipette shows any error or any mechanical fault, inform to the supplier or Engineering Department for maintenance, after repairing or maintenance, recalibrate it before use.
Download Annexure Here:
Annexure I Micropipette Calibration Record