Microscope; Purpose
To establish the procedure for ideal operation & cleaning of Microscope for use in microbiological test.
Microscope; Scope
This SOP applies for operation, cleaning and of Microscope, Model: CX21-LED SET1, Olympus, Japan in Microbiology Section at XX Pharmaceuticals Limited.
Definitions/Abbreviations
N/A
Responsibilities
The roles and responsibility is as follows
Executive/ Sr. Executive, Microbiology
Operation, cleaning and performance checking of Microscope
Manager, Microbiology
Ensure Operation, cleaning & disinfection and performance checking.
Head of Quality Assurance
Take initiative to Approval of SOP
Procedure:
Instructions
- Use 2.5% Dettol/Savlon solution for cleaning of objectives lens, eyepieces and condenser.
- Do not use any concentrate alcohol or any others solution.
- Use only immersion oil on slide.
- Wipe properly objective lens to remove immersion oil at the end of use.
- Do not disassemble the microscope without approval of Head of Microbiology Section.
Operation Procedure
- Wipe entire platform with clean cloth.
- Prepare specimen (stain, if necessary) on a glass slide & place it on stage properly under objective lens with aid of adjustment knob.
- Put on switch for light source.
- Rotating light intensity adjustment knob for increase or decrease brightness clockwise or anticlockwise.
- Adjust objective lens (10x, 40x, 100x) according to requirement.
- Use both coarse focus and then Fine focus for better visualization.
- Focus an area on the specimen, which is clearly defined or not over crowded.
- Do not move specimen holder directly by hand this will damage rotator mechanisms of the knob.
- First focus specimen with 10x then with 45x, and then with 100x objective lens (if necessary).
- After work is done wipe again lens & platform with another clean cloth, separately.
- Cover the microscope at end of the work.
Cleaning & Disinfection Procedure
- Switch off Microscope & disconnect power plug.
- Remove slide from stage.
- Clean outside of chamber with dry cloth.
- Use 2.5% Savlon/Dettol solution to Disinfect outer surfaces of Microscope
- Clean Objective lens smoothly & Ocular lens with clean, soft, fiber free cloth before and after use.
- Clean objective lens with cider wood oil if found any stain on lens.
Maintenance
If Microscope shows any mechanical, electrical or any others problem, inform to supplier or Engineering Department for corrective action.